Functional Screening for Drug Safety Testing

Cambridge Healthtech Institute’s seventh annual conference on Functional Screening for Drug Safety Testing focuses on innovative tools, assays, models and biomarkers that are being used for detecting and predicting idiosyncratic and drug-induced toxicities. This conference will bring together leading experts in drug safety, from industry, academia and government/regulatory agencies, to discuss the appropriate use of in silico, in vitro and in vivo tools, leading to informed decision-making.

Day 1 | Day 2 | Safety/Screening Brochure | Speaker Bios 

Wednesday, May 21

7:00 am Registration and Morning Coffee


IN VITRO ASSAYS FOR SAFETY TESTING 

8:00 Chairperson's Opening Remarks

Chair: Heribert Bohlen, M.D., CEO, Axiogenesis AG

 

8:05 In vitro Cardiac Safety: Current Issues and Emerging Challenges

BernardFerminiBernard Fermini, Ph.D., Ion Channel Discipline Lead, Associate Research Fellow, Global Safety Pharmacology, Pfizer Global Research & Development

Following the adoption of the S7B guidance document (2005), pharma has invested significant resources in establishing strategies to identify compounds that may cause prolongation of the QT interval. However, with increasing diversity of the portfolio, current strategies often fail to address emerging non-QT related issues, such as hypertrophy and heart failure. In this presentation we review current and future strategies to address these safety issues.

8:35 Drug Cardiotoxicity Screening by Microelectrode Arrays

SoniaGregoSonia Grego, Ph.D., Senior Research Scientist, Research Triangle Institute  (RTI) International

Driven by rapid advancement in stem cell technologies and measurement techniques, in vitro cardiotoxicity assays are under intense development. We have carried out functional screenings of iPS cardiomyocytes by a multiwell microelectrode array (MEA) system. Results will be presented of a customized analysis of the rich dataset produced by MEA for reliable extraction of multiple drug response parameters.

9:05 FEATURED PRESENTATION: Patient-Specific iPSCs for Cardiac Safety Assessments

JoeWuJoseph Wu, M.D., Ph.D., Director, Stanford Cardiovascular Institute; Professor, Department of Medicine and Radiology, Stanford
School of Medicine

Cardiac toxicity is a side effect of many pharmaceutical compounds and is a leading cause for drug withdrawal from market because of safety concerns. Current preclinical methods to measure cardiotoxicity are inefficient and rely on genetically altered cell lines, which do not accurately resemble human heart cells. Recent technological advancement has enabled the generation of patient-specific human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) in vitro. The iPSC-CMs generated in this fashion carry all the genetic information from the individuals from whom they are derived. Here I will discuss the generation of iPSC-CMs from patients with cardiovascular diseases. I will also give examples showing how iPSC-CMs can detect drug-induced cardiac toxicity more accurately than the conventional hERG testing used by most pharmaceutical companies. I will also discuss the potential applications of iPSC-CMs for drug discovery.

9:35 Coffee Break in the Exhibit Hall with Poster Viewing

10:20 FEATURED PRESENTATION: Microscale Engineered Liver Tissue for Modeling Human Disease

Sangeeta BhatiaSangeeta Bhatia M.D.,Ph.D., John J. and Dorothy Wilson Professor of Health Sciences and Technology/Institute for Medical Engineering and Science, and Professor, Electrical Engineering and Computer Science, Massachusetts Institute of Technology



10:50 Contribution of BSEP Inhibition and Mitochondrial Toxicity to Drug Induced Liver Injury - New Assays, New Insights

MichaelAleoMichael Aleo, Ph.D., Research Fellow, Pfizer Global Research & Development

In this talk I will discuss vesicle based and cell based assays for the assessment of BSEP inhibition and the learning’s we have had regarding species specificity. In addition, I will speak to the contribution of mitochondrial toxicity to DILI and will provide proof that the dual effect on BSEP and mitochondria will correlate with severe liver injury, whereas BSEP inhibition alone does not lead to liver injury per se.

11:20 Multiple Ion Channel Effects (MICE) and the New Era of Safety Pharmacology

Arthur BrownArthur M “Buzz” Brown, MD, PhD, President and CEO ChanTest Corp.

The Comprehensive in vitro Proarrhythmia Assay (CIPA) shifts the emphasis in cardiac risk assessment from measurement of QT to measurement of ventricular arrhythmias e.g., Torsade de Pointes and from measurement of hERG to measurement of cardiac ion channels and transporters that shape the cardiac action potential(MICE). A strategy for implementing, executing and validating this program will be described.


EXPLORING RELEVANT IN VIVO MODELS 

Chair: Michael Aleo, Ph.D., Research Fellow, Drug Safety, Pfizer Global Research & Development

11:50 Systems Level Approaches to Organ Specific Toxicities in Zebrafish

CalumMacRaeCalum MacRae, M.D., Ph.D., Physician-Scientist, Department of Medicine, Cardiovascular Research Center, Brigham and Women’s Hospital; Associate Professor, Harvard Medical School

Many drug effects are a result of complex phenomena that are not recapitulated even in the most sophisticated in vitro systems. Using the larval zebrafish, it is possible to model much of drug efficacy and toxicity in a native context and to do this at high throughput. We have exploited a range of assay approaches to move towards a ‘TOX’ reporter zebrafish line capable of the interrogation of core organ specific toxicities in a manner that ultimately may allow efficacy and toxicity to be balanced in the early phases of drug discovery.

12:20 pm Neurotoxicity and Cardiotoxicity in Zebrafish Embryos: Phenotype-Based Mechanistic Studies for Drug Safety Testing

Jyotshna Kanungo, Ph.D., Senior Investigator, Lead Scientist, Zebrafish HTS Laboratory, National Center for Toxicological Research, U.S. Food and Drug Administration

Manifestations of drug–drug interactions arising from the use of combination drugs could be monitored in vivo in zebrafish. Live monitoring of multiple organ/tissue toxicities is an advantage since embryos/larvae express key enzymes involved in drug metabolism including the cytochrome P450 family. Our studies show that the drug effects (alone or in combination with other drugs) in these embryos are similar to those in humans and therefore, provide an excellent platform for mechanistic studies for translatability and therapeutic intervention purposes.

12:50 Luncheon Presentation (Sponsorship Opportunity Available) or Lunch on Your Own

1:30 Session Break


EXPLORING RELEVANT IN VIVO MODELS 

2:00 Chairperson’s Remarks

Chair: Michael Aleo, Ph.D., Research Fellow, Drug Safety, Pfizer Global Research & Development

2:05 Application of Knockout Animal Models for Toxicological Safety Assessments

AndrewOlaharskiAndrew Olaharski, Ph.D., Associate Director, Toxicology, Agios Pharmaceuticals

Knockout animals are an underutilized model for assessing the safety of pharmacological targets. Despite the inherent caveats, valuable toxicological information can be gleaned from their study. Two case studies exhibiting their utility will be presented: Firstly, a host-resistance study of LRRK2 knockout rats to assess the potential immunological liabilities associated with LRRK2 inhibition and, secondly, the evaluation of selective PLK2 inhibitors in PLK2 knockout mice to assess the impact of pharmacological inhibition on causing genotoxicity.

2:35 Utilizing Mouse Population Models to Understand and Predict Drug Toxicity in Humans

Merrie MosedaleMerrie Mosedale, Ph.D., Research Scientist, Hamner-UNC Institute for Drug Safety Sciences, The Hamner Institutes for Health Sciences

Failure to accurately model clinical drug safety liabilities is, in part, due to a lack of genetic diversity present in traditional nonclinical models. Previous studies have demonstrated the ability of genetically defined mouse populations to more accurately model drug toxicity responses in humans. This presentation will describe recent findings utilizing a Mouse Diversity Panel to investigate mechanisms of drug-induced liver injury and identify risk factors that underlie drug toxicity susceptibility.

3:05 In vivo Safety Assessment of Novel Antibody-Drug Conjugates

JoergBluemelJoerg Bluemel, Ph.D., Director, Toxicology, Biologics Safety Assessment/Translational Sciences, MedImmune LLC

Antibody-drug conjugates (ADC) are complex molecules composed of a potent cytotoxic low molecular weight drug attached by a chemical linker to an antibody targeting a tumor-associated antigen. The combination of small chemical and large molecule characteristics represent a unique challenge for the design and execution of the nonclinical safety strategy. The presentation will highlight recent experience and challenges for the in vivo safety assessment of this new class of highly potent biopharmaceutical drugs.

AxioGenesis3:35 Human Cardiomyocytes - An iPSC-Derived Cell Model for Pre-Clinical Safety Studies 

Heribert Bohlen, M.D., CEO, Axiogenesis AG

Axiogenesis AG is the leading expert for high quality stem cell derived, in vitro differentiated cell types. Our ready-to-use human iPSC-derived Cor.4U cardiomyocytes display an ideal cell model for pre-clinical compound screenings and safety assay needs, due to the authentic cardiac phenotype, including normal electrophysiology, pharmacology, protein expression and morphology. Successful establishment of Cor.4U cells on LTS and HTS show the great potential of these cells to permanently substitute simple cell lines to assess cardiotoxicity.

 

4:05 Refreshment Break in the Exhibit Hall with Poster Viewing


5:00 Plenary Keynote Presentation:

Christopher AustinCatalyzing Translational Innovation

Christopher P. Austin, M.D., Director, National Center
for Advancing, Translational Sciences, National Institutes of Health

 

6:00 Networking Reception in the Exhibit Hall with Poster Viewing

7:00 Close of Day



Day 1 | Day 2 | Safety/Screening Brochure | Speaker Bios 


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Download 2014 Brochure


 

 2014 World Pharma Congress Brochure   

May 21-22


May 22-23


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